FastTools Hi-Fi PCR Kit
Fast HiFidelity Polymerase is purified from the hyper thermophilic original strain Pyrococcus spp, with super 3'-5' exonuclease activity (Proofreading activity), and high fidelity- around 80 folds of Taq DNA Polymerase. This product uses innovative affinity ligands synthesis technology, in which ligands inhibit activity of polymerase and exonuclease in a temperature dependent way. Ligand A inhibits DNA Polymerase activity at room temperature, which effectively reducing nonspecific amplification; ligand B inhibits the 3'–5' exonuclease activity and prevents the primer and template from degradation, therefore perform high specific Hot Start PCR reaction. PCR Enhancer in this product highly improves the extending speed. The PCR product is blunt-ended, could ligate with T vector after addition of “A”, or use blunt-end clone vector, as TOOLS ZBack Faster Ligation Kit (TGVTB04).
Features
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Eight times higher fidelity compared fo Pfu DNA polymerase.
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Four times higher speed compared to Taq DNA polymerase.
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Idea for GC-rich templates.
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Generates both blunt-ended and 3'-dA tailed PCR product.
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Amplification of long targets up to 15 kb form genomic DNA.